DNA

Part:BBa_K1588005:Design

Designed by: Juan David Sifontes   Group: iGEM15_UNA_Honduras   (2015-09-18)


tetravalent protein with epitopes from dengue virus to generate immune response


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1210
    Illegal PstI site found at 1363
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1210
    Illegal PstI site found at 1363
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1210
    Illegal PstI site found at 1363
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1210
    Illegal PstI site found at 1363
    Illegal NgoMIV site found at 811
    Illegal NgoMIV site found at 1621
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 890


Design Notes

1. It is coded by just one gene 2. Is not too big 3. It is exported by bacterial cells 4. Has big hydrophylic loops to insert epitopes


Source

Vibrio cholerae VC_0620

References